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OBJECTIVE:To evaluate the effects of 3 kinds of serum containing blood-activating and stasis-eliminating TCM compound formulas on aggressive behavior,Janus kinase (JAK)/signal transduction and transcriptional activator (STAT) pathway of glioma U251 cells. METHODS:Rats were randomly divided into normal saline group (5 mL/kg),Taohong Siwu decoction group(5.7 g/kg),Xuefu Zhuyu decoction(8.5 g/kg)and Didang decoction(2.8 g/kg),calculated by crude drug,intragastrically administrated once a day,for 10 d. 10% drug-containing serum culture medium was prepared after 2 h of last administration. After 10% drug-containing serum culture medium intervening U251 cells for 1 week,Transwell method was conducted to detect the cell invasion rate, Western blot was adopted to detect the metal matrix protease 2 (MMP-2), MMP-9, phosphorylated JAK2 (p-JAK2),phosphorylated STAT3(p-STAT3)protein expression;and real-time fluorescence quantitative polymerase chain reaction method was used to detect MMP-2,MMP-9 mRNA expression. RESULTS:Compared with blank serum,Xuefu Zhuyu decoction drug-containing serum can reduce cell invasion rate (P0.05). CON-CLUSIONS:In the 3 kinds of blood-activating and stasis-eliminating TCM compound formulas,Xuefu Zhuyu decoction shows sig-nificant invasive effect on inhibiting U251 cells;the mechanism may be related to inhibiting the activation of JAK2/STAT3 signal pathway and decreasing MMP-2,MMP-9 gene and protein expressions.
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Objective To investigate the epidemiological characteristics of patients with craniocerebral injury in primary level hospital of Pearl River Delta and provide evidences for developing reasonable prevention and management policy of this disease. Methods The gender,age,injury time,occupation,injury mechanism, injury information,the relation between the craniocerebral injury and the alcohol drink in the 1 898 patients with craniocerebral injury admitted to Changping People's Hospital of Dongguan in Guangdong Province were analyzed with descriptive epidemiological method. Results There were more male cases with craniocerebral injury than in female ones(1 402 cases vs. 496 cases). The major group of craniocerebral injury consisted of patients aged between 20 to 39 years old〔1088 cases(57.32%)〕. The incidence of the injury in productive workers was higher than that of employees in other kinds of work〔57.48%(1 091 cases)vs. 42.52%(807 cases)〕. The incidences of such cases were the highest between March to May〔28.71%(545 cases)〕and in October〔9.69%(184 cases)〕,December〔11.22%(213 cases)〕than those in other months. The peak days of the incidence in a week were Saturday〔392 cases(20.65%)〕and Sunday〔375 cases(19.77%)〕,but the rate of Thursday was the lowest〔201 cases(10.59%)〕. The peak time in a day was between 18 to 24 o'clock〔961 cases(50.63%)〕. Traffic accident was the leading cause of the injury accounting for 52.95%. The incidence of mild to moderate craniocerebral injury(79.98%)was much higher than that of severe and especially severe injury(20.02%). The alcohol drink was bound up with craniocerebral injury. Conclusion The occurrence of craniocerebral injury has certain regularities concerning the respects of patients' gender, age, occupation,injury time,injury mechanism,types of injury,etc and based on the epidemiological characteristics,a proper management policy should be taken to effectively reduce the morbidity of craniocerebral injury.
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Objectives To study two airway humidification methods on airway humidification of tracheotomy patients. Methods Sixty patients with traeheotomy were evenly randomized into experiment group and control group:in the former,the method of continuous oxygen atomization was used and in the latter,that of intermittent intratracheal instillation was used.The effects were compared in terms of stimulating cough,sputum clot formation,frequency of suction phlegm,time spent on the airway humidification and incidence of pulmonary infection between the groups.Results The effects in the experiment group were significantly better than in the control group in terms of stimulating cough,sputum clot formation,frequency of suction phlegm,time spent on the airway humidification and incidence of pulmonary infection(all P<0.05).Conclusion Continuous oxygen atomization can improve the effects of airway humidification,reduce the incidence of pulmonary infection and improve the efficiency of nursing work.